Journal: Human Mutation

Article Title: NDUFA4 Deletion Upregulates VDAC1 to Promote Mitochondrial Damage, Endoplasmic Reticulum Expansion, and Neuronal Apoptosis

doi: 10.1155/humu/8889386

Figure Lengend Snippet: Effects of NDUFA4 knockout on VDAC1 expression and mitochondrial‐related cellular changes. Cerebellum tissues from four pairs of wild‐type and NDUFA4 knockout mice were collected. (A) NDUFA4 and VDAC1 expression detected by Western blot. (B) Activity of ETC Complex IV measured using the MitoCheck Complex IV Activity Assay Kit. (C) Western blot analysis of cytochrome C expression in mitochondrial and cytoplasmic fractions. (D) Western blot analysis of apoptosis‐related proteins (caspase‐3, cleaved caspase‐3, caspase‐12, Bax, and Bcl‐2) and ER stress–related proteins (CHOP, ATF4, PERK, and Eif2 α ). ( n = 4). ∗ p < 0.05, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Cytoplasmic and mitochondrial proteins were isolated using the Cytoplasmic and Mitochondrial Protein Extraction Kit (C500051‐0050, Sangon Biotech).

Techniques: Knock-Out, Expressing, Western Blot, Activity Assay